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After blocking, the membranes were incubated overnight at 4 with primary antibody including anti-Nestin (Santa Cruz, sc-23,927 trail size, 1:200), anti-TUJ1 (abcam, ab105389, 1:5000), anti-VEGF-a (abcam, ab46154, 1:5000), anti-GAPDH (Servicebio, GB15002, 1:10,000), anti-iNOS (CST, 10,320, 1:2000), anti-Arg1 (abcam, ab239731, 1:5000), anti-TNF (CST, 11,948, 1:10,000), anti-IL10 (Cell Signaling Technology, 12,163, 1:5000), Axin2 (Proteintech, 20540-1-AP, 1:2000), Active -catenin (Cell Signaling Technology, 8814 S, 1:2000), -catenin (Servicebio, GB11015, 1:1000), GSK3 (abcam, ab32391, 1:2500), p-GSK3 (Abcam, ab75814, 1:2500), anti-JNK1 (Cell Signaling Technology, 9252P, 1:500), anti-p-JNK1 (Cell Signaling Technology, 9255 S, 1:500), anti-p38 (Cell Signaling Technology, 8690P, 1:500), and anti-p-p38 (Cell Signaling Technology, 4511 S, 1:500), primary antibody

50 OhtaK.OhigashiM.NaganawaA.IkedaH.SakaiM.NishikawaJ.et al (2007)