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6,7 Paraffin sections of tumours were incubated with respective primary antibodies against human -SMA (-smooth muscle actin) (1:800), cytokeratin (1:75), PCNA (proliferating cell nuclear antigen 1:200), Ki-67 (1:333), DCLK1 (1:500), E-cadherin (1:200) and vimentin (1:3000), followed by incubation with relevant secondary antibodies and DAB visualisation

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According to the Medicare NCCI Policy Manual Chapter XI, CPT 90847 may be billed on the same date as individual psychotherapy codes (90832, 90834, 90837) only if the family therapy and individual therapy are performed as separate and distinct services during separate time intervals, with each documented independently