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Till et al., 2015

BMC veterinary research , 10 , 175

Protein concentrations in lysates were determined by Protein Assay Dye Reagent (BioRad #5000006), diluted in 2x Laemmli sample buffer (BioRad Cat#1610737) with fresh -mercaptoethanol (BioRad #1610710), and heated at 95C for 5 minutes

Studies have shown antioxidants, including Glutathione, vitamin C, and vitamin E, help reduce damage from oxidative stress, improve stem cell survival, and increase the potency of stem cells