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Description

Slice preparation for electrophysiological recordings Mice were anesthetized by intraperitoneal injection of sodium pentobarbital (200 mg/kg) and were transcardially perfused with ice-cold cutting solution containing (in mM): 2.5 KCl, 1.25 NaH 2 PO 4 H 2 O, 20 HEPES, 2 Thiourea, 5 Na-ascorbate, 92 NMDG, 30 NaHCO 3 , 25 d-Glucose, 0.5 CaCl 2 2H 2 O and 10 MgSO 4 7H 2 O