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32.5 g of the powdered leaves was soaked in 100 ml of 80% ethanol and left for 72 h

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Eluted FBU-His-EEHV-EE2 samples were analysed by SDS-PAGE

The cells were seeded in 96-well plates at a density of 5,000 cells per well and incubated for 24 h at 37C and 5% CO 2