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LOXs, a family of iron-containing proteins, can catalyze the peroxidation of PUFAs on cell membranes, inducing cell death via ferroptosis

Further simulations showed that over a wide range of doxorubicin (050 M), the model can be used to suggest the specific concentration and exposure duration required to deplete cytosolic GSH by 70%, the threshold by which antioxidant defence is impaired, protein binding occurs, and cell death is possible

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Accordingly, we emphasize the need for prospective controlled studies or comparative trials to disentangle the contributions of each modality and determine whether the combination offers superior efficacy compared to monotherapy

In this assay, oxidized glutathione, produced upon reduction of peroxide substrate by glutathione peroxidase, is then recycled back to glutathione using glutathione reductase and NADPH

The hPrdx6 (1 mg/ml) at pH 7.0 and pH 4.0 was subjected to SEC, equilibrated with 3 bed volumes of 10 mM TrisHCl buffer (pH 7.0), and 10 mM sodium acetate buffer (pH 4.0), respectively