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The homogenate was filtered through a 100-M cell strainer and centrifuged at 1,000 g for 10 min at 4 C, then the nuclear pellet was washed once in nucleus preparation buffer followed by resuspension in PBS with 1% BSA and 0.2 U l Roche Protector RNase inhibitor

drafting the manuscript or revising it critically for important intellectual content

As an integrative doctor, I take a holistic approach to healthcare by treating the entire body instead of just the symptoms

Therefore, targeting the NRF2 pathway is an important strategy for treating male infertility