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Methionine is Essential amino acid for the synthesis of carrier proteins, and a lack of methionine leads to a reduction in Low-density lipoprotein synthesis and therefore the inability to transport TG out of the liver, leading to the accumulation of fatty liver [115] and steatohepatitis with similar histopathological features to human NASH [116,117,118]

RNA purification and quantitative real-time PCR Porcine granulosa cells were harvested and extracted for total RNA using a RNA extraction kit (TaKaRa, Dalian, China) according to the manufacturers protocols

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doi: 10.1136/pmj.79.930.218

10.1016/j.ultsonch.2023.106313 116 ManzoorM