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Cells expressing cytosolic or mitochondrial Grx1-roGFP2 were seeded at equal densities in standard culture medium without phenol red in -Slide eight-well ibiTreat microscopy chambers (Ibidi, Munich, Germany)

& Puga, I

With that regulatory brake reduced, IGF-1 LR3 interacts with IGF-1 receptors more directly and for a longer duration, which is why research protocols report a half-life of roughly 2030 hours, compared to native IGF-1s 1215 hours