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For the experimental procedures, Caco-2 cells were subcultured and utilized at passages 510 in DMEM containing 10% FBS and 1% penicillin/streptomycin and incubated at 37 C in a humidified atmosphere with 5% CO

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Moreover, tobacco use is responsible for 71% of all lung cancer deaths and 42% of all chronic obstructive pulmonary diseases

[10] The 1971 NRHP nomination says "Descendants of the Halls (among whom were the McNeelys and the Strachans from whom comes the present name of the house) have lived there for over a hundred years." [3] Hall's descendants continued to live in the house until 1972, at which time the Historic Salisbury Foundation bought it along with some original furnishings

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