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PRAS40 antagonizes GF-dependent mTORC1 activation 37,38,39,40

(PubMed) Guilarte TR, Wagner HN, Jr., Frost JJ

About this article Cite this article Minari, T.P., Pisani, L.P

Personality & Emotional Intelligence 00:51:28 Sponsor: LMNT 00:52:40 Texting & Relationships 01:00:37 Tool: Mood Meter, Energy & Pleasantness Scale 01:06:28 Emotion Suppression

Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0