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The inclusion of a hexahistidine-tag in sdAbs (and other recombinant proteins) initially serves purification purposes (57, 58), however, it also offers the advantage of easy radiolabeling with 99m Tc through tricarbonyl chemistry ( 99m Tc proves to be an ideal radioisotope for the design of bimodal sdAb tracers given its wide availability via 99 Mo/ 99m Tc-generators, its half-life aligning well with the blood half-life of sdAbs (49), its relatively low radiation energy profile enhancing the safety for both patients and personnel, and its routine use for gamma-probing in the operating theatre, meaning all hardware, protocols and experience is available

Lipid-polymer bilaminar oxygen nanobubbles for enhanced photodynamic therapy of cancer

doi:10.1002/jbm.a.34420 128

On top of that, multiple studies have shown that erythron is involved in additional functions

To confirm that the difference in the peak shift was mainly due to phase transition instead of the effect of temperature, DLPC liposomes were immobilized at 25 C and 50 C