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All animals were housed under controlled temperature and a 12-hour lightdark cycle, with ad libitum access to food and water

AEEI-2021-058), and conducted in accordance with ethical requirements

The mixtures of FolinCiocalteau reagent and sodium carbonate solution were combined with the OIFE and gallic acid solutions, followed by incubating in darkness for 2 h at room temperature and assessing in triplicate

Standard cycling involves 4-8 weeks of use followed by 2-4 weeks off before resuming if needed

Fibrosis is a highly dynamic process associated with overactivated fibroblasts in organs, such as the heart, lung and kidney 8,38,39

710 3 cells were pre-adhered to 96-well plate