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Viability of spheroids were determined using CellTiter-Glo 3D cell viability assay kit (Promega)

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CBS filament formation in cells To investigate CBS filamentation in situ, we employed fluorescence microscopy across various cell lines, including non-transformed (MEF, hFB), prostate cancer (PC-3), and breast cancer (BT549, MCF7, MDA-MB-231) cells (Fig