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Another study found that rats with Cytosolic Iron-Sulfur Cluster Assembly (CIA) had much higher amounts of free radicals and protease activity than normal rats

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Conclusions Ultimately, the current maintenance dose of 1,000 mcg B12 every 3 months may confer long term harm, therefore it seems that once B12 levels are stabilised well within acceptable range, then the standard B12 intervention of 1000 mcg should be administered annually, and monitored with pre-injection annual testing of levels