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Influence of chemical elements on mammalian spermatozoa

Supplementary material The Supplementary Material for this article can be found online at: Glossary CGA Chlorogenic acid IC50 half-maximal inhibitory concentration WB Western blotting RT-qPCR real-time quantitative polymerase chain reaction ROS reactive oxygen species NAC N-Acetyl-L-cysteine Nrf2 nuclear factor erythroid 2-related factor 2 LD 50 semi-lethal concentration CQA caffeoylquinic acid CA caffeic acid QA quinic acid DPPH 1,1-diphenyl-2-picrylhydrazyl radical ANIT alpha-naphthylisothiocyanate APAP Acetaminophen EGFR Epidermal growth factor receptor ALP alkaline phosphatase ALT alanine aminotransferase EGR1 early growth response-1 iNOS inducible nitric oxide synthase GO gene ontology KEGG Kyoto Encyclopedia of Genes and Genomes DMEM Dulbeccos Modified Eagle Medium FBS fetal bovine serum CCK8 Cell Counting Kit-8 RTCA real-time cell analysis LDH lactate dehydrogenase LFQ-MS label-free quantitative mass spectrometry ALP alkaline phosphatase OCR oxygen consumption rate ECAR extracellular acidification rate FCCP mitochondrial oxidative phosphorylation uncoupler c-Myc Myc proto-oncogene protein MAPK mitogen-activated protein kinase Erk1/2 extracellular signal-regulated kinase 1/2 STAT3 signal transducer and activator of transcription 3 GSH glutathione GSSG glutathione disulfide PBS phosphate buffered saline ICP-MS inductively coupled plasma mass spectrometry KM mice Kunming mice CTSD cathepsin D CAPN1 calpain 1 CYCS cytochrome c CCl 4 Carbon tetrachloride EIF2S1 eukaryotic translation initiation factor 2 subunit TfR1 transferrin receptor 1 GPX4 glutathione peroxidase 4 SLC7A11 cystine/glutamate transporter SOD superoxide dismutase Chac1 glutathione-specific gamma-glutamylcyclotransferase 1 RRM2 ribonucleoside-diphosphate reductase subunit M2 GSR glutathione reductase GCLM glutamate--cysteine ligase regulatory subunit TM4SF1 transmembrane 4 L6 family member 1 PGD 6-phosphogluconate dehydrogenase G6PD glucose-6-phosphate 1-dehydrogenase GSTP1 glutathione S-transferase P IREB2 Iron-responsive element-binding protein 2 ALDH1B1 mitochondrial aldehyde dehydrogenase X ACADVL very long-chain specific acyl-CoA dehydrogenase CAPE Caffeic acid phenethyl ester DFO deferoxamine ESCC esophageal squamous cell carcinoma

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Niedelman, Wendy