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DFO was also able to prevent ferroptosis in the IB3-1 CF AECs, presumably through its ability to chelate ferric iron safely and circumvent the generation of redox active ferrous iron

The second step was the introduction of the enzyme collagenase into the liver lobes disrupting the supporting extracellular matrix

The supernatants were collected by centrifugation at 5000 rpm for 5 min at 4 C and precleared by incubated with GFP-Trap agarose beads (Chromotek) or HA-Beads (Roche) for 2 h at 4 C with rotation